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Johan Decelle

Dr Johan Decelle, PhD
Laboratoire Physiologie Cellulaire & Végétale
CEA-Grenoble
17 avenue des Martyrs
38 054 Grenoble cedex 09
France
Phone: 04 38 78 28 69
Phone: 04 38 78 28 69

Research project: Metabolic interactions in planktonic photosymbioses

The project aims at investigating the metabolic interactions in planktonic photosymbioses using a combination of omics approaches and high-resolution imaging. Photosymbiosis between single-celled hosts and microalgae is common in oceanic plankton, especially in oligotrophic surface waters. However, the functioning and physiology of this ecologically important cell-cell interaction and the subcellular mechanisms allowing the host to accommodate and benefit from its endosymbionts remain enigmatic. The project will address different research questions: How does a heterotrophic cell take control and domesticate a photosynthetic cell? What are the structural and metabolic controls? What are the metabolic services provided by the chloroplast to a host? Different models of photosymbiosis in unicellular organisms will be studied in order to compare mechanisms on an evolutionary perspective: the oceanic symbiosis between radiolarians and microalgae widely distributed in the oceans, and the freshwater symbiotic association between the ciliate Paramecium and the microalga Chlorella.

The project will mainly use a combination of quantitative single-cell structural and chemical imaging techniques (3D electron microscopy, nanoSIMS, Synchrotron X-ray fluorescence) to study the structural organization, physiology and trophic status of the microalgal cells in symbiosis within their host and in free-living grown in culture medium. Complementary approaches such as metabolomics, in vivo photophysiology and proteomics will also be conducted to have a comprehensive understanding of metabolic interactions.
The project is supported for 3 years by a Chair of Excellence (IDEX-Labex GRAL) of the University of Grenoble Alpes in France.

Symbiosis between Acantharia (unicellular host) and intracellular microalgae (yellow cells) is widespread and abundant in the surface ocean. The host builds a complex mineral skeleton.


Collaborators:
- Sophie Marro from the Laboratoire d'Océanographie de Villefranche CNRS UMR 7093 (Villefranche-sur-Mer, France).
- Benoît Gallet from Institut de Biologie Structurale (IBS) - UMR5075 - Electron Microscopy and Methods (Grenoble, France).
- Ian Probert from the Roscoff Marine Station - Culture Collection (Roscoff, France).
- Giulia Veronesi from CNRS – CEA Grenoble and the European Synchrotron Radiation Facility (ESRF-Grenoble).
- Yannick Schwab and Nicole Schieber from the EMBL (Heidelberg).
- Niculina Musat and Hryhoriy Stryhanyuk from ProVIS - Helmholtz Centre for Environmental Research – UFZ Department of Isotope Biogeochemistry (Leipzig, Germany).


Curriculum vitae

Since June 2018 Junior team leader. Chair of Excellence University of Grenoble Alpes (IDEX). Plant and Cell Physiology Laboratory- CEA Grenoble (France).
2016 - 2018 Post-Doctoral Fellow (Marie Sklodowska Curie fellowship)
Helmholtz Centre for Environmental Research – UFZ Department of Isotope Biogeochemistry - ProVIS Team
2013 - 2015 Post-Doctoral Fellow
University Pierre et Marie Curie Paris VI - CNRS, UMR7144, Station Biologique de Roscoff, France
2009 - 2012 PhD
University Pierre et Marie Curie Paris VI - CNRS, UMR7144, Station Biologique de Roscoff, France
2008 Research Assistant
EMBL Heidelberg, Germany
2006 - 2007 Master Sc. - Integrative Biology and Physiology - University Pierre et Marie Curie Paris VI (UPMC), France
2002 - 2005 B.Sc. in Biology
University Pierre et Marie Curie Paris VI (UPMC), France

Field of expertise
- Molecular ecology and evolution of marine protists
- Phylogeny, metabarcoding
- Sample preparation for electron microscopy (SEM and TEM) and chemical imaging (ToF-SIMS and nanoSIMS) applied to single celled eukaryotes
- Symbioses in marine plankton


Publications (available on ResearchGate)

    Decelle J, Carradec Q, Pochon X, Henry N, Romac S, Mahé F, Dunthorn M, Kourlaiev A, Voolstra C.R., Winker P, and de Vargas C (2018) Worldwide occurrence and activity of the reef-building coral symbiont Symbiodinium in the open ocean. Current Biology 28

    Meng M, Marchet C, Corre E, Peterlongo P, Alberti A, Da Silva C, Wincker P, Pelletier E, Probert I, Decelle J, Le Crom S, Not F and BittnerL. (2018) A de novo approach to disentangle partner identity and function in holobiont systems. Microbiome 6:105.

    Jiang H, Kilburn MR, Decelle J, Musat N (2016) NanoSIMS chemical imaging combined with correlative microscopy for biological sample analysis. Current Opinion in Biotechnology 41: 130-135

    Mordret S, Romac S, Henry N, Colin S, Carmichael M, Berney C, Audic S, Richter DJ, Pochon X, de Vargas C, Decelle J(2015) The symbiotic life of Symbiodinium in the open ocean within a new species of calcifying ciliate (Tiarina sp.). The ISME journal doi:10.1038/ismej.2015.211
    De Vargas C, Audic S, Henry N, Decelle J, Mahé F, Romac S et al. (2015) Eukaryotic plankton diversity in the sunlit ocean. Science. 348: 1261605
    Lima-Mendez G, Faust K, Henry N, Decelle J, Colin S et al. (2015) Determinants of community structure in the global plankton interactome. Science. 348: 1262073
    Decelle J and Not F (2015) Acantharia. Encyclopedia of Life Sciences
    Decelle J, Christen R, Romac S, Stern RF, Bendif EM, Zingone A et al. (2015) PhytoREF: a reference database of the plastidial 16S rRNA gene of photosynthetic eukaryotes with curated taxonomy. Molecular Ecology Resources 15: 1435-1445
    Balzano S, Corre E, Decelle J, Sierra R, Wincker P, Da Silva C, Poulain J, Pawlowski J, Not F (2015) Transcriptome analyses to investigate symbiotic relationships between marine protists. Frontiers in microbiology 6 doi: 10.3389/fmicb.2015.00098
    Biard T, Suzuki N, Pillet L, Poirier C, Decelle J, Not F (2015) Integrative taxonomy of the Collodaria (Polycystinea, Radiolaria). Protist 166: 374-388
    Andersen RA, Bailey JC, Decelle J, Probert I (2015) Phaeocystis rex sp. nov. (Phaeocystales, Prymnesiophyceae): a new solitary species that produces a multilayered scale cell covering. European Journal of Phycology 50: 207-222
    Decelle J, Romac S, Sasaki E, Not F, Mahé F (2014) Intracellular diversity of the V4 and V9 regions of the 18S rRNA in marine protists (Radiolarians) assessed by high-throughput sequencing. PLoS ONE 9(8):e104297
    Probert I, Siano R, Poirier C, Decelle J, Biard T, Tuji A, Suzuki N, Not F (2014) Brandtodinium gen. nov. and B. nutricula comb. Nov. (Dinophyceae), a dinoflagellate commonly found in symbiosis with polycystine radiolarians. Journal of Phycology 50: 388-399
    Decelle J (2013) New perspectives on the functioning and evolution of photosymbiosis in plankton: Mutualism or parasitism? Communicative & Integrative Biology 6:4 (Invited article)
    Decelle J, Martin P, Paborstava K, Pond DW, Tarling G, Mahé F, de Vargas C, Lampitt R, Not F (2013) Diversity, ecology and biogeochemistry of cyst-forming Acantharia (Radiolaria) in the oceans. PLoS ONE 8(1): e53598
    Lejzerowicz F, Esling P, Majewski W, Szczucinski W, Decelle J, Obadia C, Martinez-Arbizu P, Pawlowski J (2013) Ancient DNA complements microfossil record in deep-sea subsurface sediments. Biology Letters 9 (4): 20130283
    Sierra R, Matz MV, Aglyamova G, Pillet L, Decelle J, Not F, de Vargas C, Pawlowski J (2013) Deep relationships of Rhizaria revealed by phylogenomics: A farewell to Haeckel’s Radiolaria. Molecular Phylogenetics and Evolution 67: 53-59
    Guillou L, Bachar D, Audic S, Bass D, Berney C, Bittner L, Boutte C, Burgaud G, de Vargas C, Decelle J, del Campo J, Dolan JR, Dunthorn M, Edvardsen B, Holzmann M, Kooistra W, Lara E, Le Bescot N, Logares R, Mahe F, Massana R, Montresor M, Morard R, Not F, Pawlowski J, Probert I, Sauvadet AL, Siano R, Stoeck T, Vaulot D, Zimmermann P and Christen R. (2013) The Protist Ribosomal Reference database (PR2): a catalog of unicellular eukaryote Small Sub-Unit rRNA sequences with curated taxonomy. Nucleic Acids Research 41: 597-604
    Decelle J, Probert I, Bittner L, Desdevises Y, Colin S, de Vargas, Galí M, Simó R, Not F (2012) An original mode of symbiosis in open ocean plankton. Proceedings of the National Academy of Sciences (PNAS) 109: 18000-18005
    Decelle J, Siano R, Probert I, Poirier C, Not F (2012) Multiple microalgal partners in symbiosis with the Acantharia Acanthochiasma sp. (Radiolaria). Symbiosis 58: 233-244
    Decelle J, Suzuki N, Mahé F, de Vargas C, Not F (2012) Molecular phylogeny and morphological evolution of the Acantharia (Radiolaria) Protist 163: 435-450
    Pawlowski J, Adl S, Audic S, Bass D, Belbahri L, Berney C, Bowser SS, Cepicka I, Decelle J, Dunthorn M, Fiore-Donno AM, Gile GH, Holzmann M, Jahn R, Jirku M, Keeling PJ, Kostka M, Kudryavtsev A, Lara E, Lukeš J, Mann DG, Mitchell EAD, Nitsche F, Romeralo M, Saunders GW, Simpson AGB, Smirnov AV, Spouge J, Stern RF, Stoeck T, Zimmermann J, Schindel D, de Vargas C. (2012) CBOL Protist Working Group: Barcoding eukaryotic richness beyond the animal, plant and fungal kingdoms. PLoS Biology 10(11): e1001419
    Karsenti E et al. (2011) A holistic approach to marine eco-systems biology. PLoS Biology, 9(10): e1001177
    Decelle J, Andersen AC, Hourdez S (2010) Morphological adaptations to chronic hypoxia in deep-sea decapod crustaceans from hydrothermal vents and cold seeps. Marine Biology 157: 1259-1269

Ph.D thesis:
Evolution and Ecology of photosymbiosis in Acantharia (Radiolaria).
PhD defense, December 2012 with Prof. David A. Caron from the University of Southern California, Los Angeles, (USA) and Dr. Fabrice Vavre from the University of Lyon (France) as main referees "rapporteur".

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