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Symbiosis 3D imaging

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Published on 5 January 2021
Missions
The main objective of the project is to develop high-resolution 3D imaging in "cryo" mode on single-celled photosymbioses in marine plankton (symbiosis between a host cell and intracellular microalgae). More specifically, the aim of the candidate will be to develop and apply a sample preparation protocol with correlative microscopy, including cryofixation and tomographic imaging (FIB-SEM and cryo-TEM). Part of the project will also include image analysis to reveal the subcellular architecture of photosymbioses.

Activities
- Implementation of a workflow in cryo mode for 3D electron microscopy
- Image analysis (segmentation, 3D reconstruction)
- Cryo fluorescence microscopy
- Communication through international meetings and workshops.

Skills
- Cell biology or biophysics using electron and/or correlative microscopy.
- Knowledge and expertise in sample preparation for 3D electron microscopy (FIB-SEM, cryo-TEM) and fluorescence microscopy in cryo mode. Expertise in cryoFIB-SEM will be appreciated.
- OR (not required) Knowledge and expertise in image analysis for 3D reconstruction after FIB-SEM or cryo-TEM.
- Ability to work autonomously in an interdisciplinary and international environment, and to interact in a young and dynamic research group.

Work context
The candidate will be part of the Photosymbiosis team (photosymbiosis.com) of the Laboratory of Cell and Plant Physiology (UMR 5168 - CNRS -CEA - Univ. Grenoble Alpes - INRA) within the IRIG institute of the CEA of Grenoble. The team's projects aim to better understand the functioning of unicellular photosymbioses by combining subcellular microscopy approaches (nanoSIMS, X-Ray fluorescence synchrotron, 3D electron microscopy). Locally, the project will be in close collaboration with the IRIG institute: INAC platform and the Institute of Structural Biology (IBS, Grenoble). This position is part of an international project (SymbioChip) led by the Station Biologique de Roscoff and funded by The Gordon and Betty Moore Foundation (more info here). The candidate will interact regularly with the project partners during meetings and field sampling: Station Biologique de Roscoff (CNRS-Sorbonne Univ), Bigelow Laboratory for Ocean Sciences, and the University of Jena Friedrich-Schiller.

General information
Reference: UMR5168-JOHDEC-002
Workplace: GRENOBLE
Date of publication: Tuesday, December 22, 2020
Type of Contract: FTC Scientist
Contract Period: 18 months
Expected date of employment: 1st April 2021
Proportion of work: Full time
Remuneration: gross monthly salary 2648-3054 euros
Desired level of education: 5-year university degree
Experience required: Indifferent