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Technician in genomic engineering and lipidomics of a marine diatom

Descriptif en français.
Published on 31 January 2020
The Laboratory of Cell and Plant Physiology (Laboratoire de Physiologie Cellulaire & Végétale; Interdisciplinary Research Institute in Grenoble – IRIG – CEA) opens a position in the Lipid team to work on a specific group of algae called the diatoms. The selected technician will contribute the characterisation of genes involved in the synthesis of lipids in the marine diatom Phaeodactylum tricornutum. Diatoms are unicellular photosynthetic organisms and P. tricornutum is considered the model organism for diatom biology and physiology, as well as for development of biotechnological approaches. A large number of molecular tools are available for P. tricornutum, like genetic transformation and genomic engineering based on nucleases such as TALEN and CRISPR/Cas9. Gene knock out or knock down will be performed to produce mutants partially or totally deprived of one or more genes of interest. A lipidomic phenotypic characterisation will lead to the characterisation of the functions of the enzymes encoded by the aforementioned genes.
The candidate will be involved in the construction of expression vectors for genomic engineering and in the genetic transformation of such vectors into P. tricornutum via biolistics. The candidate will take care of the genetic as well as phenotypic screening of the mutant strains by means of a screening procedure recently established in the lab. Promising strains will be analysed for their lipid content by GC-MS and LC-MS/MS in order to characterise the functions of the genes.
A percentage of the time of the candidate will be devoted to a collaborative project with a private company, which aims at improving lipid production in different marine protist.

Required qualifications
Diploma equivalent to Licence professionnelle, with special options on biotechnologies, microbiology, plant biology, cell culture, molecular biology and/or biochemistry.

Required skills
• Data manipulation and reporting.
• Molecular cloning.
• Axenic algal or other microorganism cultivation.
• Effective written and oral communication skills.
• Ability to work independently and in a group.
• Abilitiy to contribute to multiple projects.
• Ability to learn new topics and think critically and creatively for problem solving.

Further information
18 months, possible 24 months depending on conditions.
Start as soon as possible.
Contact: send a curriculum vitae, cover letter and references to Eric Maréchal and Alberto Amato

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